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During animal development, a complex multicellular organism is formed from a single cell - the fertilized egg. This process is regulated through differential gene expression. Specific non-coding DNA elements called enhancers regulate gene transcription during animal development. The Kvon lab uses a combination of novel genomics tools, genome editing, and mouse developmental biology techniques to understand transcriptional regulation by enhancers in the context of mammalian development, evolution, and human congenital disorders.